Anti-HP1 alpha Antibody [ARC0244] (A308742)

Name: Anti-HP1 alpha Antibody [ARC0244]
Brand: Antibodies.com
SKU: A308742
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0244] antibody to HP1 alpha for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Heterochromatin Marker

Specifications

Name

Anti-HP1 alpha Antibody [ARC0244]

Description

Rabbit monoclonal [ARC0244] antibody to HP1 alpha.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 92-191 of human HP1 alpha/CBX5 (P45973).

Sequence

SNFSNSADDIKSKKKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLMKWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWHAYPEDAENKEKETAKS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0244

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

22 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen p25, CBX5, CBX5_HUMAN, CG8409, Chromobox 5, Chromobox homolog 5, Chromobox homolog 5 (HP1 alpha homolog, Drosophila), Chromobox protein homolog 5, Epididymis luminal protein 25, HEL25, Heterochromatin protein 1, Heterochromatin protein 1 alpha, Heterochromatin protein 1 homolog alpha, HP1, HP1 alpha homolog, HP1A, HP1Hs alpha, Su(var)205

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-HP1 alpha Antibody [ARC0244] (A308742)

Western blot analysis of extracts of various cell lines, using Anti-HP1 alpha Antibody [ARC0244] (A308742) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-HP1 alpha Antibody [ARC0244] (A308742)

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Figure 3: Immunohistochemistry - Anti-HP1 alpha Antibody [ARC0244] (A308742)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-HP1 alpha Antibody [ARC0244] (A308742) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HP1 alpha Antibody [ARC0244] (A308742)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-HP1 alpha Antibody [ARC0244] (A308742) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.