Anti-HP1 alpha Antibody (A88817)

Name: Anti-HP1 alpha Antibody
Brand: Antibodies.com
SKU: A88817
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to HP1 alpha for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Heterochromatin Marker

Specifications

Name

Anti-HP1 alpha Antibody

Description

Rabbit polyclonal antibody to HP1 alpha.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human HP1 alpha/HP1 alpha/CBX5 (NP_001120794.1).

Sequence

MGKKTKRTADSSSSEDEEEYVVEKVLDRRVVKGQVEYLLKWKGFSEEHNTWEPEKNLDCPELISEFMKKYKKMKEGENNKPREKSESNKRKSNFSNSADDIKSKKKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLMKWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWHAYPEDAENKEKETAKS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

22 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen p25, CBX5, CBX5_HUMAN, CG8409, Chromobox 5, Chromobox homolog 5, Chromobox homolog 5 (HP1 alpha homolog, Drosophila), Chromobox protein homolog 5, Epididymis luminal protein 25, HEL25, Heterochromatin protein 1, Heterochromatin protein 1 alpha, Heterochromatin protein 1 homolog alpha, HP1, HP1 alpha homolog, HP1A, HP1Hs alpha, Su(var)205

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-HP1 alpha Antibody (A88817)

Western blot analysis of extracts of various cell lines, using Anti-HP1 alpha Antibody (A88817) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-HP1 alpha Antibody (A88817)

Immunohistochemistry analysis of paraffin-embedded rat hippocampus using Anti-HP1 alpha Antibody (A88817) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-HP1 alpha Antibody (A88817)

Immunohistochemistry analysis of paraffin-embedded human brain astrocytoma using Anti-HP1 alpha Antibody (A88817) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HP1 alpha Antibody (A88817)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-HP1 alpha Antibody (A88817) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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