Horse POMC ELISA Kit (A326186)

Horse POMC ELISA Kit

Horse POMC ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of horse POMC in serum, plasma, saliva or other biological fluids.

Sandwich (quantitative)

Horse POMC ELISA Kit (A326186) employs the sandwich enzyme immunoassay technique for the quantitative measurement of horse POMC in serum, plasma, saliva or other biological fluids. An antibody specific for POMC has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the POMC present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-POMC Antibody, which binds the captured POMC present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of POMC captured in each well. The concentration of POMC can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colourmetric

Colorimetric Microplate Reader

45 pg/ml

125-8000 pg/ml

4h 30m

Horse

Intra-assay: CV < 10%, Inter-assay: CV < 12%

Information online is representative. Always refer to the Product Manual inside the kit.

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

This product is for research use only. It is not intended for diagnostic or therapeutic use.