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Anti-HN1 Antibody (A88464)

Western Blot - Anti-HN1 Antibody (A88464) - Antibodies.com Immunohistochemistry - Anti-HN1 Antibody (A88464) - Antibodies.com Immunohistochemistry - Anti-HN1 Antibody (A88464) - Antibodies.com Immunohistochemistry - Anti-HN1 Antibody (A88464) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to HN1 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-HN1 Antibody
Description
Rabbit polyclonal antibody to HN1.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human HN1 (NP_057269.1).
Sequence
MTTTTTFKGVDPNSRNSSRVLRPPGGGSNFSLGFDEPTEQPVRKNKMASNIFGTPEENQASWAKSAGAKSSGGREDLESSGLQRRNSSEASSGDFLDLKGEGDIHENVDTDLPGSLGQSEEKPVPAAPVPSPVAPAPVPSRRNPPGGKSSLVLG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
16 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Androgen regulated protein 2, Androgen-regulated protein 2, ARM2, Hematological and neurological expressed 1, Hematological and neurological expressed 1 protein, HN1A, HN1_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-HN1 Antibody (A88464) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-HN1 Antibody (A88464)
Western blot analysis of extracts of HT-29 cells, using Anti-HN1 Antibody (A88464) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-HN1 Antibody (A88464) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-HN1 Antibody (A88464)
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-HN1 Antibody (A88464) at a dilution of 1:150 (20x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-HN1 Antibody (A88464) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-HN1 Antibody (A88464)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-HN1 Antibody (A88464) at a dilution of 1:150 (20x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-HN1 Antibody (A88464) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-HN1 Antibody (A88464)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-HN1 Antibody (A88464) at a dilution of 1:150 (20x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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