Anti-HMGB2 Antibody [ARC1460] (A307358)

Name: Anti-HMGB2 Antibody [ARC1460]
Brand: Antibodies.com
SKU: A307358
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1460] antibody to HMGB2 for WB, IHC, ICC/IF, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-HMGB2 Antibody [ARC1460]

Description

Rabbit monoclonal [ARC1460] antibody to HMGB2.

Applications

WB, IHC, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, CUT&Tag: 1⁶ cells/1µg

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGB2 (P26583).

Sequence

MGKGDPNKPRGKMSSYAFFVQTCREEHKKKHPDSSVNFAEFSKKCSERWKTMSAKEKSKFEDMAKSDKARYDREMKNYVPPKGDKKGKKKDPNAPKRPPS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1460

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

24 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C80539, High mobility group (nonhistone chromosomal) protein 2, High mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2, HMG B2, HMG-2, HMG2, HMGB2_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Anti-HMGB2 Antibody [ARC1460] (A307358)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1µg of Anti-HMGB2 Antibody [ARC1460] (A307358), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of HMGB2 in representative gene loci (PTBP1), as shown in figure.

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Figure 2: Western Blot - Anti-HMGB2 Antibody [ARC1460] (A307358)

Western blot analysis of extracts of various cell lines, using Anti-HMGB2 Antibody [ARC1460] (A307358) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-HMGB2 Antibody [ARC1460] (A307358)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-HMGB2 Antibody [ARC1460] (A307358) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HMGB2 Antibody [ARC1460] (A307358)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-HMGB2 Antibody [ARC1460] (A307358) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.