Anti-HMGB1 Antibody [1F3] (A85355)

Name: Anti-HMGB1 Antibody [1F3]
Brand: Antibodies.com
SKU: A85355
Price: 185.0 USD
Availability: InStock
Rating: 5 (1 reviews)

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$185

Mouse monoclonal [1F3] antibody to HMGB1 for WB, ICC/IF and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-HMGB1 Antibody [1F3]

Description

Mouse monoclonal [1F3] antibody to HMGB1.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000-1:2,000, ICC/IF: 1:1,000, IHC: 1:1,000

Reactivity

Human, Rat, Mouse

Immunogen

Full-length recombinant human HMGB1 protein, expressed in and purified from E. coli.

Host

Mouse

Clonality

Monoclonal

Clone ID

1F3

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

The HMGB1 protein is normally located in the nucleus where it associates with chromatin and DNA and, as such, this antibody is a useful nuclear marker.

Synonyms

Amphoterin, Chromosomal protein, nonhistone, HMG1, DKFZp686A04236, High mobility group 1, High mobility group box 1, High mobility group protein 1, High mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, HMG-1, HMG1, HMG3, HMGB 1, HMGB1_HUMAN, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP 1, Sulfoglucuronyl carbohydrate binding protein

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunofluorescence - Anti-HMGB1 Antibody [1F3] (A85355)

Immunofluorescent analysis of of HeLa cells stained with Anti-HMGB1 Antibody [1F3] (A85355), dilution 1:2,000, in red and co-stained with Anti-GAPDH Antibody (A85377), dilution 1:2,000 in green. Anti-HMGB1 Antibody [1F3] stains the chromatin binding protein HMGB1, which is localized in the nuclei. In contrast the Anti-GAPDH Antibody produces strong cytoplasmic staining of cells.

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Figure 2: Western Blot - Anti-HMGB1 Antibody [1F3] (A85355)

Western blot analysis of lysates from different cell lines probed with Anti-HMGB1 Antibody [1F3] (A85355), dilution 1:2,000. The lanes contain: [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y cell lystates. The 25 kDa band revealed by Anti-HMGB1 Antibody [1F3] corresponds to the HMGB1 protein.

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