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Anti-HMGA1 Antibody (A88672)

Western Blot - Anti-HMGA1 Antibody (A88672) - Antibodies.com Immunohistochemistry - Anti-HMGA1 Antibody (A88672) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to HMGA1 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-HMGA1 Antibody
Description
Rabbit polyclonal antibody to HMGA1.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1).
Sequence
MSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEEGISQ
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
20 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
High mobility group (nonhistone chromosomal) protein isoforms I and Y, High mobility group AT hook 1, High mobility group AT-hook protein 1, High mobility group protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein Ia, High mobility group protein R, HMG R, HMG-I(Y), HMGA1A, HMGA1_HUMAN, HMGIY, MGC12816, MGC4242, MGC4854, Nonhistone chromosomal high mobility group protein HMG I/HMG Y
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-HMGA1 Antibody (A88672) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-HMGA1 Antibody (A88672)
Western blot analysis of extracts of 293T cells, using Anti-HMGA1 Antibody (A88672) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry - Anti-HMGA1 Antibody (A88672) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-HMGA1 Antibody (A88672)
Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-HMGA1 Antibody (A88672) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Publishing research using Anti-HMGA1 Antibody (A88672)? Please let us know so that we can list the citation on this page.

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