This antibody reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Applications
WB, IHC-P, IHC-Fr
Dilutions
IHC-P: 1:60 to 1:100, incubation: 1 h at RT; Positive Control: human placenta - extravillous cytotrophoblast, heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven), WB: Recommended dilution 1-2 µg/ml.
Reactivity
Human
Immunogen
Denatured bacterially expressed recombinant human HLA-G heavy chain.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-G/1
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, HLA-6.0, HLAG, MHC class I antigen G
Immunohistochemistry - Anti-HLA G Antibody [MEM-G/1] (A86276)
Immunohistochemistry staining with Anti-HLA G Antibody (A86276) - pulmonary disseases (paraffin-embedded sections). Anti-HLA G Antibody (A86276) stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.