Anti-HLA DRA Antibody [IPO-10] (A248847)

Anti-HLA DRA Antibody [IPO-10]

Mouse monoclonal [IPO-10] antibody to HLA DRA.

This antibody defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37, and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnosis of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, and RPMI-8226 (50% of cells). This antibody does not label T cell lines, blood granulocytes, thymocytes, or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This antibody labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. This antibody reacts with AMML cells and in a majority of Hodgkins disease cases a significant percentage of affected lymph node cells were detected.

Flow Cytometry, IF

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Human, Monkey

Spleen cells of a patient with hairy cell leukemia (Daudi cells).

Mouse

Monoclonal

IPO-10

IgG3

kappa

Protein A/G chromatography.

200 µg/ml

~36 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Store at 4°C.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-HLA DRA Antibody [IPO-10] - BSA and Azide free (A252027).

DR alpha chain, DR alpha chain precursor, DRA_HUMAN, DRB1, DRB4, Histocompatibility antigen HLA DR alpha, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DR alpha chain, HLA DR1B, HLA DR3B, HLA DRA1, HLA DRB1, HLA DRB3, HLA DRB4, HLA DRB5, HLA-DR, HLA-DRA, HLADR4B, HLADRA1, HLADRB, Major histocompatibility complex class II DR alpha, Major histocompatibility complex class II DR beta 1, Major histocompatibility complex class II DR beta 3, Major histocompatibility complex class II DR beta 4, Major histocompatibility complex class II DR beta 5, MGC117330, MHC cell surface glycoprotein, MHC class II antigen DRA, MHC II, MLRW