Anti-HLA DR Antibody [SPM289] (A277642)

Anti-HLA DR Antibody [SPM289]

Mouse monoclonal [SPM289] antibody to HLA DR.

This antibody reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 0.5-1 µg/ml, IHC-P: 1-2 µg/ml, IHC-Fr: 1-2 µg/ml

Human

Activated human peripheral blood mononuclear cells.

Mouse

Monoclonal

SPM289

IgG3

Unconjugated

Protein A/G chromatography.

200 µg/ml

~28 kDa (beta chain)

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-HLA DR Antibody [SPM289] - BSA and Azide free (A278230).

HLA DP, HLA DR + HLA DP, MHC Class II