The antibody recognises MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.
Applications
Flow Cytometry, IP, IHC-Fr, ICC, ELISA, CyTOF
Dilutions
Flow Cytometry: 1-12 µg/ml.
Reactivity
Bovine, Human, Non-Human Primates, Feline
Cross Reactivity
This antibody does not cross react with Rabbit.
Immunogen
Membrane of human tonsil cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
W6/32
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
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