Anti-HLA Class I Antibody [MEM-147] (PE) (A85471)

Mouse monoclonal [MEM-147] antibody to HLA Class I (PE) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-HLA Class I Antibody [MEM-147] (PE)
Description
Mouse monoclonal [MEM-147] antibody to HLA Class I (PE).
Specificity
The antibody recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-2 µg/ml.
Reactivity
Human
Immunogen
PHA-activated peripheral blood lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-147
Isotype
IgG1
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
DKFZp686P19218, EA1.2, EA2.1, HLA 5.4, HLA 6.2, HLA A, HLA B, HLA C, HLA CDA12, HLA F, HLA G, HLA G histocompatibility antigen class I G, HLAF, Major histocompatibility complex class I, Major histocompatibility complex class I A, Major histocompatibility complex class I E, Major histocompatibility complex class I F, MHC, MHC Class I antigen, MHC G
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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