Anti-HLA Class 1 ABC Antibody [Bra-23/9] (A315084)

Anti-HLA Class 1 ABC Antibody [Bra-23/9]

Mouse monoclonal [Bra-23/9] antibody to HLA Class 1 ABC.

Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Immunoprecipitation of lactoperoxidase radioiodinated cell surface proteins and surface sialoglycoproteins radiolabeled by sodium metaperiodate/tritiated sodium borohydride technique confirmed the structure gp44,p12 (consisting of a 44kDa glycopeptide linked with a non-glycosylated 12kDa peptide) typical for MHC class I antigen(s) as a structure recognized by Bra23/9. Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.

Flow Cytometry, IF

THP-1 and Jurkat cells. Tonsil or lymph node.

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Human, Mouse, Non-Human Primates

Human REH cells (pre-B leukaemia cell line).

Mouse

Monoclonal

Bra-23/9

IgG2a

Unconjugated

Protein A/G chromatography.

200 µg/ml

~41 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

HLA A, HLA B, HLA C, HLA class 1 A, HLA class 1 B, HLA class 1 C, Major histocompatibility complex, class I, A + B + C, MHC class I HLA A, MHC class I HLA B, MHC class I HLA C, MHC HLA ABC