This antibody recognizes the HLA-B27 cell surface antigen on human cells. It may be used to HLA type human lymphocytes. Approximately 60% of patients with ankylosing spondylitis are HLA-B27 positive. This reagent can be used to help identify this HLA haplotype in human lymphocytes. Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind pep- tides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B encodes a membrane anchored heavy chain, which hetero-dimerizes with a light chain (-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B alleles. The HLA-B27 allele appears with increased frequency in uveitis patients.

Applications

Flow Cytometry, IF

Dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Reactivity

Human

Immunogen

Lymphocytes from an HLA-B27 patient.

Host

Mouse

Clonality

Monoclonal

Clone ID

EP-4

Isotype

IgM

Light Chains

kappa

Conjugate

Unconjugated

Concentration

200 µg/ml

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-HLA B Antibody [EP-4] - BSA and Azide free (A254325).

Synonyms

1B07_HUMAN, AS, B-4901, B-7 alpha chain, HC class I antigen SHCHA, HLA class I histocompatibility antigen, HLA class I histocompatibility antigen, B alpha chain, HLA-B, HLA-B histocompatibility type, HLAB, leukocyte antigen class I-B, major histocompatibility complex, class I, B, MHC class 1 antigen, MHC class I antigen B*7, MHC class I antigen HLA-B alpha chain, MHC class I antigen HLA-B heavy chain, MHC Class I HLA heavy chain, MHC class I molecule, MHC HLA-B cell surface glycoprotein, MHC HLA-B transmembrane glycoprotein, SPDA1

Isotype Controls