Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Name: Anti-Histone H3.3 Antibody [ARC0255]
Brand: Antibodies.com
SKU: A306483
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0255] antibody to Histone H3.3 for WB, IHC, IP, ChIP and ChIP-seq.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Histone H3.3 Antibody [ARC0255]

Description

Rabbit monoclonal [ARC0255] antibody to Histone H3.3.

Applications

WB, IHC, IP, ChIP, ChIP-seq

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, ChIP-seq: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 57-136 of human Histone H3.3 (P84243).

Sequence

KSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0255

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H3 histone family 3A, H3 histone family 3B, H3 histone, family 3B (H3.3B), H3.3, H3.3A, H3.3B, H33_HUMAN, H3F3, H3F3A, H3f3b, Histone H3.3Q, Histone H3.A, Histone H3.B, MGC87782, MGC87783

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Chromatin Immunoprecipitation - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Chromatin immunoprecipitations analysis of cross-linked chromatin from HeLa cells, using Anti-Histone H3.3 Antibody [ARC0255] (A306483). The ChIP sequencing results indicate the enrichment pattern of Histone H3. 3 in selected genomic region and representative gene loci (GAPDH), as shown in figure.

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Figure 2: Western Blot - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Western blot analysis of extracts of Rat lung, using Anti-Histone H3.3 Antibody [ARC0255] (A306483) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Western Blot - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Western blot analysis of extracts of various cell lines, using Anti-Histone H3.3 Antibody [ARC0255] (A306483) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 4: Immunohistochemistry - Anti-Histone H3.3 Antibody [ARC0255] (A306483)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Histone H3.3 Antibody [ARC0255] (A306483) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.