Anti-Histone H2A.X (phospho Ser139) Antibody (A16444)

Western blot analysis of 293T, using Anti-Histone H2A.X (phospho Ser139) Antibody (A16444) at 1:500 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Western blot analysis of NIH/3T3, using Anti-Histone H2A.X (phospho Ser139) Antibody (A16444) at 1:500 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Western blot analysis of C6, using Anti-Histone H2A.X (phospho Ser139) Antibody (A16444) at 1:500 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.