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Recombinant Anti-Histone H2AX Antibody [RM214] (A121318)

Western Blot - Anti-Histone H2AX Antibody [RM214] (A121318) - Antibodies.com Immunocytochemistry - Anti-Histone H2AX Antibody [RM214] (A121314) - Antibodies.com
Recombinant Antibody
$565

Recombinant rabbit monoclonal [RM214] antibody to Histone H2AX for WB, ELISA, Multiplex Protein Detection and ICC.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Histone H2AX Antibody [RM214]
Description
Recombinant rabbit monoclonal [RM214] antibody to Histone H2AX.
Specificity
This antibody recognizes Histone H2AX protein, independent of post-translational modifications.
Applications
WB, ELISA, Multiplex Protein Detection, ICC
Dilutions
WB: 0.5-2 µg/ml, ICC: 1-2 µg/ml, ELISA: 0.2-1 µg/ml, Multiplex Protein Detection: 0.2-1 µg/ml
Reactivity
All Vertebrates
Cross Reactivity
There is no cross reactivity with other Histone proteins.
Immunogen
Synthetic peptide corresponding to human Histone H2AX (C terminal).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM214
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AW228881, H2A histone family member X, H2A.FX, H2A.X, H2a/x, H2AFX, H2AX, H2AX histone, H2AX_HUMAN, Hist5.2ax, Histone 2A, Histone 2AX, Histone H2A.X, RGD1566119
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Histone H2AX Antibody [RM214] (A121318) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Histone H2AX Antibody [RM214] (A121318)
Western blot of A375, HEK293, HeLa, and SK-MEL-2 whole cell lysates, using Anti-Histone H2AX Antibody [RM214] at 0.5µg/ml, showed endogenous Histone H2AX in A375, HEK293, HeLa, and SK-MEL-2 cells.
Immunocytochemistry - Anti-Histone H2AX Antibody [RM214] (A121314) - Antibodies.comEnlarge Image
Figure 2: Immunocytochemistry - Anti-Histone H2AX Antibody [RM214] (A121318)
Immunocytochemistry of HeLa cells, using Anti-Histone H2AX Antibody [RM214] (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Publishing research using Recombinant Anti-Histone H2AX Antibody [RM214] (A121318)? Please let us know so that we can list the citation on this page.

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