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Anti-Histone H2AX Antibody (A80512)

Name
Anti-Histone H2AX Antibody
Description
Rabbit polyclonal antibody to Histone H2AX.
Applications
WB, IHC, IF
Dilutions
WB: 1:500-1:2000, IHC: 1:100-1:200, IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human H2AFX (NP_002096.1).
Sequence
VYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQEY
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
16kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AW228881, H2A histone family member X, H2A.FX, H2A.X, H2a/x, H2AFX, H2AX, H2AX histone, H2AX_HUMAN, Hist5.2ax, Histone 2A, Histone 2AX, Histone H2A.X, RGD1566119
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Histone H2AX Antibody (A11361) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Histone H2AX Antibody (A80512)
Western blot analysis of extracts of various cell lines, using Anti-Histone H2AX Antibody (A11361) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunohistochemistry - Anti-Histone H2AX Antibody (A11361) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Histone H2AX Antibody (A80512)
Immunohistochemistry of paraffin-embedded human stomach using Anti-Histone H2AX Antibody (A11361) at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-Histone H2AX Antibody (A11361) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Histone H2AX Antibody (A80512)
Immunohistochemistry of paraffin-embedded rat heart using Anti-Histone H2AX Antibody (A11361) at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-Histone H2AX Antibody (A11361) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Histone H2AX Antibody (A80512)
Immunohistochemistry of paraffin-embedded mouse brain using Anti-Histone H2AX Antibody (A11361) at dilution of 1:100 (40x lens).
Publishing research using Anti-Histone H2AX Antibody (A80512)? Please let us know so that we can list the citation on this page.

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