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Epigenetics & Nuclear Signaling
Histones
Variants
Anti-Histone H2A.X (phospho Ser139) Antibody (A93597)
Overview
Specifications
Images
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$185
Product Datasheet
Rabbit polyclonal antibody to Histone H2A.X (phospho Ser139) for WB and ELISA.
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100µg
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$40 to United States
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5-8 Business Days
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Specifications
Name
Anti-Histone H2A.X (phospho Ser139) Antibody
Description
Rabbit polyclonal antibody to Histone H2A.X (phospho Ser139).
Specificity
This antibody detects endogenous levels of H2AFX only when phosphorylated at Ser139.
Applications
WB
,
ELISA
Dilutions
WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide derived from human Histone H2A.X around the phosphorylation site of Ser139.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
15kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Rabbit IgG (A82272)
Rabbit IgG (A17360)
Suitable Secondaries
Goat Anti-Rabbit IgG H&L Antibody (AP) (A294874)
Goat Anti-Rabbit IgG H&L Antibody (Biotin) (A294795)
Goat Anti-Rabbit IgG H&L Antibody (FITC) (A294887)
Goat Anti-Rabbit IgG H&L Antibody (HRP) (A294888)
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (2)
Enlarge Image
Figure 1: Anti-Histone H2A.X (phospho Ser139) Antibody (A93597)
ELISA for immunogen phosphopeptide (left) and non-phosphopeptide (right) using Anti-Histone H2A.X (phospho Ser139) Antibody.
Enlarge Image
Figure 2: Western Blot - Anti-Histone H2A.X (phospho Ser139) Antibody (A93597)
Western blot analysis of lysates from 293 cells treated with heat shock using Anti-Histone H2A.X (phospho Ser139) Antibody.
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