Anti-Histone H2A.X Antibody [ARC0590] (A80673)

Name: Anti-Histone H2A.X Antibody [ARC0590]
Brand: Antibodies.com
SKU: A80673

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This product is discontinued

Anti-Histone H2A.X Antibody [ARC0590] (A80673) has been discontinued and is no longer available.

View all Histone H2A.X Antibodies.

Specifications

Name

Anti-Histone H2A.X Antibody [ARC0590]

Description

Rabbit monoclonal [ARC0590] antibody to Histone H2A.X.

Applications

WB, IHC, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2AX (P16104).

Sequence

MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0590

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H2a/x, H2AFX, H2AX, Histone H2AX

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Histone H2A.X Antibody [ARC0590] (A80673)

Western blot analysis of extracts of various cell lines, using Anti-Histone H2A.X Antibody [ARC0590] (A80673) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-Histone H2A.X Antibody [ARC0590] (A80673)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Histone H2A.X Antibody [ARC0590] (A80673) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H2A.X Antibody [ARC0590] (A80673)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Histone H2A.X Antibody [ARC0590] (A80673) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H2A.X Antibody [ARC0590] (A80673)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Histone H2A.X Antibody [ARC0590] (A80673) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.