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Anti-Histone H2A.X Antibody (A13852)

Western Blot - Anti-Histone H2A.X Antibody (A13852) - Antibodies.com Immunofluorescence - Anti-Histone H2A.X Antibody (A13852) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Histone H2A.X for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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orders@antibodies.com

Name
Anti-Histone H2A.X Antibody
Description
Rabbit polyclonal antibody to Histone H2A.X.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around S2 of human Histone H2AX (NP_002096.1).
Sequence
MSGRG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
15 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AW228881, H2A histone family member X, H2A.FX, H2A.X, H2a/x, H2AFX, H2AX, H2AX histone, H2AX_HUMAN, Hist5.2ax, Histone 2A, Histone 2AX, Histone H2AX, RGD1566119
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Histone H2A.X Antibody (A13852) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Histone H2A.X Antibody (A13852)
Western blot analysis of extracts of various cell lines, using Anti-Histone H2A.X Antibody (A13852) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-Histone H2A.X Antibody (A13852) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Histone H2A.X Antibody (A13852)
Confocal immunofluorescence analysis of HeLa cells using Anti-Histone H2A.X Antibody (A13852) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Histone H2A.X Antibody (A13852)? Please let us know so that we can list the citation on this page.

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