Histone H1 (phospho Thr17) Cell Based ELISA Kit (A103768)

Name: Histone H1 (phospho Thr17) Cell Based ELISA Kit
Brand: Antibodies.com
SKU: A103768
Price: 645.0 USD
Availability: InStock

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$645

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Freight Charges

$40 to United States

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5-8 Business Days

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Specifications

Name

Histone H1 (phospho Thr17) Cell Based ELISA Kit

Assay Type

Cell-based (qualitative)

Principle of Assay

This Histone H1 (phospho Thr17) Cell Based ELISA Kit allows for the detection of Histone H1 (phospho Thr17) and the effects that certain stimulation conditions have on Histone H1 (phospho Thr17) expression in different cell lines. Qualitative determination of Histone H1 (phospho Thr17) concentration is achieved by an indirect ELISA format. In essence, Histone H1 (phospho Thr17) is captured by Anti-Histone H1 (phospho Thr17) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-Histone H1 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Platform

Microplate

Detection Type

Fluorometric

Product Range

> 5000 Cells

Reactivity

Human, Mouse

Recovery

Storage

Store at + 4°C. Stable for 6 months.

Synonyms

dJ221C16.2, dJ221C16.5, DmeH1, H1, H1 0 histone, H1 histone family, H1 histone family member 0, H1 histone family member 1, H1 histone family member 3, H1 histone family member 5, H1 histone family member N testis specific, H1 histone family member O oocyte specific, H1 histone family member X, H1(0), H1.1, H1.2, H1.3, H1.4, H1.5, H10, H14_HUMAN, H1A, H1f0, H1F0 protein, H1F1, H1F2, H1F3, H1F4, H1F5, H1FNT, H1FOO, H1FOO protein, H1FOO_HUMAN, H1FT, H1FV, H1FX, H1oo, H1t, H1T2, H1X, HANP1, His1, HisC, HIST1, HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E, HIST1H1T, Histone, Histone 1 H1a, Histone 1 H1b, Histone 1 H1c, Histone 1 H1d, Histone 1 H1e, Histone 1 H1t, Histone H1', Histone H1.0, Histone H1.1, Histone H1.2, Histone H1.3, Histone H1.4, Histone H1.5, Histone H1a, Histone H1B, Histone H1oo, Histone H1x, MGC116819, MGC126630, MGC126632, MGC126642, MGC138176, MGC138345, MGC15959, MGC3992, MGC50807, MGC5241, MGC8350, Oocyte specific histone H1, Oocyte specific linker histone H1, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1, Testicular H1 histone

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Histone H1 (phospho Thr17) Cell Based ELISA Kit (A103768)

Western blot analysis of extracts from Jurkat cells treated with UV 15', using Anti-Histone H1 (phospho Thr17) Antibody.

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Figure 2: Histone H1 (phospho Thr17) Cell Based ELISA Kit (A103768)

A diagram showing the protocol for Histone H1 (phospho Thr17) Cell Based ELISA Kit.

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Figure 3: Histone H1 (phospho Thr17) Cell Based ELISA Kit (A103768)

A summary of the protocol for Histone H1 (phospho Thr17) Cell Based ELISA Kit.

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Figure 4: Western Blot - Histone H1 (phospho Thr17) Cell Based ELISA Kit (A103768)

The mouse monoclonal antibody to GAPDH is used as a positive control in Histone H1 (phospho Thr17) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.

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