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Histone H1 Cell Based ELISA Kit (A103376)

Western Blot - Histone H1 Cell Based ELISA Kit (CB5332) - Antibodies.com Protocol Diagram - Histone H1 Cell Based ELISA Kit (CB5332) - Antibodies.com Protocol Summary - Histone H1 Cell Based ELISA Kit (CB5332) - Antibodies.com Western Blot - Control Antibody - Histone H1 Cell Based ELISA Kit (CB5332) - Antibodies.com
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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Histone H1 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This Histone H1 Cell Based ELISA Kit allows for the detection of Histone H1 and the effects that certain stimulation conditions have on Histone H1 expression in different cell lines. Qualitative determination of Histone H1 concentration is achieved by an indirect ELISA format. In essence, the Histone H1 is captured by Anti-Histone H1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Histone H1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
dJ221C16.2, dJ221C16.5, DmeH1, H1, H1 0 histone, H1 histone family, H1 histone family member 0, H1 histone family member 1, H1 histone family member 3, H1 histone family member 5, H1 histone family member N testis specific, H1 histone family member O oocyte specific, H1 histone family member X, H1(0), H1.1, H1.2, H1.3, H1.4, H1.5, H10, H14_HUMAN, H1A, H1f0, H1F0 protein, H1F1, H1F2, H1F3, H1F4, H1F5, H1FNT, H1FOO, H1FOO protein, H1FOO_HUMAN, H1FT, H1FV, H1FX, H1oo, H1t, H1T2, H1X, HANP1, His1, HisC, HIST1, HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E, HIST1H1T, Histone, Histone 1 H1a, Histone 1 H1b, Histone 1 H1c, Histone 1 H1d, Histone 1 H1e, Histone 1 H1t, Histone H1', Histone H1.0, Histone H1.1, Histone H1.2, Histone H1.3, Histone H1.4, Histone H1.5, Histone H1a, Histone H1B, Histone H1oo, Histone H1x, MGC116819, MGC126630, MGC126632, MGC126642, MGC138176, MGC138345, MGC15959, MGC3992, MGC50807, MGC5241, MGC8350, Oocyte specific histone H1, Oocyte specific linker histone H1, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1, Testicular H1 histone
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Histone H1 Cell Based ELISA Kit (CB5332) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Histone H1 Cell Based ELISA Kit (A103376)
Western blot analysis of extracts from COLO cells, using Anti-Histone H1 (Ab-17) Antibody.
Protocol Diagram - Histone H1 Cell Based ELISA Kit (CB5332) - Antibodies.comEnlarge Image
Figure 2: Histone H1 Cell Based ELISA Kit (A103376)
A diagram showing the protocol for Histone H1 Cell Based ELISA Kit.
Protocol Summary - Histone H1 Cell Based ELISA Kit (CB5332) - Antibodies.comEnlarge Image
Figure 3: Histone H1 Cell Based ELISA Kit (A103376)
A summary of the protocol for Histone H1 Cell Based ELISA Kit.
Western Blot - Control Antibody - Histone H1 Cell Based ELISA Kit (CB5332) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Histone H1 Cell Based ELISA Kit (A103376)
The mouse monoclonal antibody to GAPDH is used as a positive control inHistone H1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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