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Anti-Histone H1.2 Antibody [ARC1836] (A307750)

Western Blot - Anti-Histone H1.2 Antibody [ARC1836] (A307750) - Antibodies.com Western Blot - Anti-Histone H1.2 Antibody [ARC1836] (A307750) - Antibodies.com Immunofluorescence - Anti-Histone H1.2 Antibody [ARC1836] (A307750) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC1836] antibody to Histone H1.2 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Histone H1.2 Antibody [ARC1836]
Description
Rabbit monoclonal [ARC1836] antibody to Histone H1.2.
Applications
WB, ICC/IF
Dilutions
WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H1.2 (P16403).
Sequence
MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTG
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1836
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
32 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
H1 histone family member 2, H1.a, H12_HUMAN, H1F2, H1s-1, HIST1H1C, Histone 1 H1c, Histone cluster 1 H1c, Histone H1c, Histone H1d, Histone H1s-1, MGC3992
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Histone H1.2 Antibody [ARC1836] (A307750) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Histone H1.2 Antibody [ARC1836] (A307750)
Western blot analysis of extracts of various cell lines, using Anti-Histone H1.2 Antibody [ARC1836] (A307750) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Histone H1.2 Antibody [ARC1836] (A307750) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Histone H1.2 Antibody [ARC1836] (A307750)
Western blot analysis of extracts of various cell lines, using Anti-Histone H1.2 Antibody [ARC1836] (A307750) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunofluorescence - Anti-Histone H1.2 Antibody [ARC1836] (A307750) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Histone H1.2 Antibody [ARC1836] (A307750)
Immunofluorescence analysis of U-2 OS cells using Anti-Histone H1.2 Antibody [ARC1836] (A307750) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Histone H1.2 Antibody [ARC1836] (A307750)? Please let us know so that we can list the citation on this page.

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