Anti-Histone H1.0 Antibody [ARC1059] (A307908)

Name: Anti-Histone H1.0 Antibody [ARC1059]
Brand: Antibodies.com
SKU: A307908
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1059] antibody to Histone H1.0 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Histone H1.0 Antibody [ARC1059]

Description

Rabbit monoclonal [ARC1059] antibody to Histone H1.0.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H1.0 (P07305).

Sequence

MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1059

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H1 histone family member 0, H1(0), H10, H10_HUMAN, h1f0, H1FV, Histone H1'', Histone H1(0), Histone H10, Histone H5, MGC5241, N-terminally processed

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Histone H1.0 Antibody [ARC1059] (A307908)

Western blot analysis of extracts of various cell lines, using Anti-Histone H1.0 Antibody [ARC1059] (A307908) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Histone H1.0 Antibody [ARC1059] (A307908)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Histone H1.0 Antibody [ARC1059] (A307908) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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