Anti-Histone H1.0 Antibody (A14423)

Name: Anti-Histone H1.0 Antibody
Brand: Antibodies.com
SKU: A14423
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to Histone H1.0 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-Histone H1.0 Antibody

Description

Rabbit polyclonal antibody to Histone H1.0.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human H1F0 (NP_005309.1).

Sequence

MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDEPKKSV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H1 histone family member 0, H1(0), H10, H10_HUMAN, h1f0, H1FV, Histone H1'', Histone H1(0), Histone H10, Histone H5, MGC5241, N-terminally processed

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Histone H1.0 Antibody (A14423)

Western blot analysis of extracts from normal (control) and Histone H1.0 knockout (KO) 293T cells, using Anti-Histone H1.0 Antibody (A14423) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Histone H1.0 Antibody (A14423)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Histone H1.0 Antibody (A14423) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Histone H1.0 Antibody (A14423)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Histone H1.0 Antibody (A14423) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Histone H1.0 Antibody (A14423)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Histone H1.0 Antibody (A14423) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.