Recombinant Anti-His Tag Antibody [RM146] (Biotin) (A121257)

Name: Anti-His Tag Antibody [RM146] (Biotin)
Brand: Antibodies.com
SKU: A121257
Price: 510.0 USD
Availability: InStock

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$510

Recombinant rabbit monoclonal [RM146] antibody to His Tag (Biotin) for WB, IP, ICC, Flow Cytometry and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Recombinant Antibody

Specifications

Name

Anti-His Tag Antibody [RM146] (Biotin)

Description

Recombinant rabbit monoclonal [RM146] antibody to His Tag (Biotin).

Specificity

This antibody recognizes recombinant proteins containing the 6 x His Tag or 10 x His Tag, fused to either the amino or carboxy terminus.

Applications

WB, IP, ICC, Flow Cytometry, IHC

Dilutions

WB: 0.1-0.5 µg/ml, ICC: 0.5-2 µg/ml, FC: 0.5-2 µg/ml, IHC: 0.1-1 µg/ml, ELISA: 0.01-0.5 µg/ml

Reactivity

All Species

Cross Reactivity

There is no cross reactivity with other endogenous protein in mammalian or bacteria cells.

Immunogen

Mixture containing peptide with His Tag (N terminal) and peptide with His Tag (C terminal).

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM146

Isotype

IgG

Conjugate

Biotin

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Anti-His Tag Antibody [RM146] (Biotin) (A121257)

A titer ELISA performed on purified recombinat PCSK9 protein (His tag). A serial dilution of Anti-His Tag Antibody [RM146] (Biotin) was used as the primary antibody, followed by alkaline phosphatase conjugated Streptavidin.

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Figure 2: Western Blot - Anti-His Tag Antibody [RM146] (Biotin) (A121257)

Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding Histone H3 (G34W) protein (His tag), using Anti-His Tag Antibody [RM146] (Biotin) at 0.2µg/ml, followed by a HRP conjugated Streptavidin.

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Figure 3: Immunohistochemistry - Anti-His Tag Antibody [RM146] (Biotin) (A121257)

Immunohistochemistry staining of naive HepG2 cells (Negative control) using Anti-His Tag Antibody [RM146] (Biotin).

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Figure 4: Immunohistochemistry - Anti-His Tag Antibody [RM146] (Biotin) (A121257)

Immunohistochemistry staining of 293T cells expressing His Tag nuclear protein X, using Anti-His Tag Antibody [RM146] (Biotin).

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