Recombinant Anti-His Tag Antibody [r6HIS/6423] (A277941)

Anti-His Tag Antibody [r6HIS/6423]

Recombinant mouse monoclonal [r6HIS/6423] antibody to His Tag.

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of hexa-histidine. This MAb is highly specific to hexa-histidine and shows no cross-reaction with other structurally related compounds. It has a very high affinity for hexa-histidine and is excellent for use in various techniques. His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein s biochemical properties. A variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest.

WB, Flow Cytometry, IF, IP

WB: 1-2 µg/ml, Flow Cytometry: 0.5-1 µg/million cells, IF: 0.5-1 µg/ml, IP: 2-4µg / 500µg proteins

Hexa-histidine tagged recombinant human CD70 protein.

Mouse

Monoclonal

r6HIS/6423

IgG1

Unconjugated

Protein A/G chromatography.

200 µg/ml

244Da

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-His Tag Antibody [r6HIS/6423] - BSA and Azide free (A278529).

6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag