Anti-His Tag Antibody [ARC2696] (A309752)

Name: Anti-His Tag Antibody [ARC2696]
Brand: Antibodies.com
SKU: A309752

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This product is discontinued

Anti-His Tag Antibody [ARC2696] (A309752) has been discontinued and is no longer available.

View all His Tag Antibodies.

Specifications

Name

Anti-His Tag Antibody [ARC2696]

Description

Rabbit monoclonal [ARC2696] antibody to His Tag.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:2,000-1:6,000, ICC/IF: 1:50-1:200, IP: 1:10,000-1:30,000

Reactivity

Species Independent

Immunogen

A synthetic peptide corresponding to His tag.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2696

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-His Tag Antibody [ARC2696] (A309752)

Western blot analysis of extracts of His Protein, using Anti-His Tag Antibody [ARC2696] (A309752) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunofluorescence - Anti-His Tag Antibody [ARC2696] (A309752)

Immunofluorescence analysis of 293T-SOST His(C) and 293T using Anti-His Tag Antibody [ARC2696] (A309752) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-His Tag Antibody [ARC2696] (A309752)

Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-His Tag Antibody [ARC2696] (A309752). This Western blot was performed on the immunoprecipitate using Anti-His Tag Antibody [ARC2696] (A309752) at a dilution of 1:30000.