This antibody recognises the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Goat anti-Histidine tag antibody may not detect all N-terminal histidine tagged proteins. Goat anti-Histidine tag antibody has been shown to be suitable for use on cytospins.
Applications
WB, ELISA
Dilutions
ELISA 1: 1:1,000 - 1:30,000
Reactivity
Species Independent
Immunogen
Keyhole Limpet Haemocyanin conjugated to 6x His
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.
Synonyms
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag