Figure 1: Western Blot - Anti-His Tag Antibody [AMC0149] (A17313)
Western blot analysis of various lysates, using Anti-His Tag Antibody [AMC0149] (A17313) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Figure 2: Immunoprecipitation - Anti-His Tag Antibody [AMC0149] (A17313)
Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using Anti-His Tag Antibody [AMC0149] (A17313). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
Figure 3: Western Blot - Anti-His Tag Antibody [AMC0149] (A17313)
Immunoprecipitation analysis of 300µg extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-tag using 3µg of Anti-His Tag Antibody [AMC0149] (A17313). This Western blot was performed on the immunoprecipitate using Anti-His Tag Antibody [AMC0149] (A17313)at 1:5,000 dilution.
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