This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of hexa-histidine. This antibody is highly specific to hexa-histidine and shows no cross-reaction with other structurally related compounds.
Figure 1: SDS-PAGE - Anti-His Tag Antibody [6HIS/3550] (A250872)
SDS-PAGE analysis of Anti-His Tag Antibody [6HIS/3550] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.