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Anti-HIP2/LIG Antibody (A83244)

Immunohistochemistry - Anti-HIP2/LIG Antibody (A83244) - Antibodies.com Western Blot - Anti-HIP2/LIG Antibody (A83244) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to HIP2/LIG for ELISA, WB and IHC.

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$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-HIP2/LIG Antibody
Description
Goat polyclonal antibody to HIP2/LIG.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:2,000, WB: 1-3 µg/ml, IHC: 1-2µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C terminal region of human HIP2/LIG.
Sequence
C-SWDVETATELLLSN
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
24 kDa
Predicted MW
22.4 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - Anti-HIP2/LIG Antibody (A83244) - Antibodies.comEnlarge Image
Figure 1: Immunohistochemistry - Anti-HIP2/LIG Antibody (A83244)
HIP2/LIG expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-HIP2/LIG Antibody (A83244) at 3.8µg/ml and revealed with alkaline phosphatase (AP).
Western Blot - Anti-HIP2/LIG Antibody (A83244) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-HIP2/LIG Antibody (A83244)
HIP2/LIG expression in Jurkat cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-HIP2/LIG Antibody (A83244) at 1.5µg/ml and detected by chemiluminescence.
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