Anti-HIF1 beta Antibody [ARC0010] (A306522)

Name: Anti-HIF1 beta Antibody [ARC0010]
Brand: Antibodies.com
SKU: A306522
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0010] antibody to HIF1 beta for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-HIF1 beta Antibody [ARC0010]

Description

Rabbit monoclonal [ARC0010] antibody to HIF1 beta.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 499-789 of human HIF-1 beta (P27540).

Sequence

LAPRQQQQQTELDMVPGRDGLASYNHSQVVQPVTTTGPEHSKPLEKSDGLFAQDRDPRFSEIYHNINADQSKGISSSTVPATQQLFSQGNTFPPTPRPAENFRNSGLAPPVTIVQPSASAGQMLAQISRHSNPTQGATPTWTPTTRSGFSAQQVATQATAKTRTSQFGVGSFQTPSSFSSMSLPGAPTASPGAAAYPSLTNRGSNFAPETGQTAGQFQTRTAEGVGVWPQWQGQQPHHRSSSSEQHVQQPPAQQPGQPEVFQEMLSMLGDQSNSYNNEEFPDLTMFPPFSE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0010

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

87 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

arnT, ARNT protein, ARNT_HUMAN, Aryl hydrocarbon receptor nuclear translocator, bHLHe2, Class E basic helix-loop-helix protein 2, Dioxin receptor, Dioxin receptor nuclear translocator, Drnt, HIF 1 beta, HIF 1beta, HIF-1-beta, HIF1-beta, HIF1B, HIF1beta, Hypoxia Inducible Factor 1, Hypoxia inducible factor 1 beta, Hypoxia-inducible factor 1-beta, nuclear translocator, Tango

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-HIF1 beta Antibody [ARC0010] (A306522)

Western blot analysis of extracts of HeLa cells, using Anti-HIF1 beta Antibody [ARC0010] (A306522) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-HIF1 beta Antibody [ARC0010] (A306522)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-HIF1 beta Antibody [ARC0010] (A306522) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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