Anti-Hexokinase 1 Antibody [ARC0256] (A306070)

Name: Anti-Hexokinase 1 Antibody [ARC0256]
Brand: Antibodies.com
SKU: A306070
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0256] antibody to Hexokinase 1 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Knockout Validated Antibody Mitochondrial Outer Membrane Marker

Specifications

Name

Anti-Hexokinase 1 Antibody [ARC0256]

Description

Rabbit monoclonal [ARC0256] antibody to Hexokinase 1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hexokinase 1 (P19367).

Sequence

GLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0256

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BB404130, Brain form hexokinase, dea, DrHXK1, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, hexokinase I, Hexokinase PI, Hexokinase type I, Hexokinase, tumor isozyme, Hexokinase-1, Hexokinase-A, HK I, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, HXK1_HUMAN, im:7148527, mHk1-s, wu:fc09d08, wu:fc16e02, wu:fc21e02, wu:fq14b11, zgc:55790, zgc:77618

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Hexokinase 1 Antibody [ARC0256] (A306070)

Western blot analysis of extracts from wild type (WT) and HK1 knockout (KO) 293T cells, using Anti-Hexokinase 1 Antibody [ARC0256] (A306070) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-Hexokinase 1 Antibody [ARC0256] (A306070)

Western blot analysis of extracts of various cell lines, using Anti-Hexokinase 1 Antibody [ARC0256] (A306070) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-Hexokinase 1 Antibody [ARC0256] (A306070)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Hexokinase 1 Antibody [ARC0256] (A306070) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Hexokinase 1 Antibody [ARC0256] (A306070)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Hexokinase 1 Antibody [ARC0256] (A306070) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.