Anti-Hexokinase 1 Antibody (A12985)

Name: Anti-Hexokinase 1 Antibody
Brand: Antibodies.com
SKU: A12985
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to Hexokinase 1 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody Mitochondrial Outer Membrane Marker

Specifications

Name

Anti-Hexokinase 1 Antibody

Description

Rabbit polyclonal antibody to Hexokinase 1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-219 of human HK1 (NP_000179.2).

Sequence

KIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

102 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BB404130, Brain form hexokinase, dea, DrHXK1, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, hexokinase I, Hexokinase PI, Hexokinase type I, Hexokinase, tumor isozyme, Hexokinase-1, Hexokinase-A, HK I, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, HXK1_HUMAN, im:7148527, mHk1-s, wu:fc09d08, wu:fc16e02, wu:fc21e02, wu:fq14b11, zgc:55790, zgc:77618

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Hexokinase 1 Antibody (A12985)

Western blot analysis of extracts of various cell lines, using Anti-Hexokinase 1 Antibody (A12985) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Hexokinase 1 Antibody (A12985)

Western blot analysis of extracts from normal (control) and HK1 knockout (KO) 293T cells, using Anti-Hexokinase 1 Antibody (A12985) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-Hexokinase 1 Antibody (A12985)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Hexokinase 1 Antibody (A12985) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Hexokinase 1 Antibody (A12985)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Hexokinase 1 Antibody (A12985) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.