+1 (314) 370-6046 or
Contact Us
Explore our End of Year Promotion >>
Antibodies.com LogoAntibodies.com Logo
Sign In / RegisterMy Account
0

HER2 (phospho Tyr1248) Cell Based ELISA Kit (A103792)

Western Blot - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573) - Antibodies.com Protocol Diagram - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573) - Antibodies.com Protocol Summary - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573) - Antibodies.com Western Blot - Control Antibody - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573) - Antibodies.com
$645
100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
HER2 (phospho Tyr1248) Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This HER2 (phospho Tyr1248) Cell Based ELISA Kit allows for the detection of HER2 (phospho Tyr1248) and the effects that certain stimulation conditions have on HER2 (phospho Tyr1248) expression in different cell lines. Qualitative determination of HER2 (phospho Tyr1248) concentration is achieved by an indirect ELISA format. In essence, HER2 (phospho Tyr1248) is captured by Anti-HER2 (phospho Tyr1248) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-HER2 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Platform
Microplate
Detection Type
Fluorometric
Product Range
> 5000 Cells
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
C erb B2/neu protein, CD340, CD340 antigen, Cerb B2/neu protein, CerbB2, Erb b2 receptor tyrosine kinase 2, ErbB 2, ERBB2, ERBB2_HUMAN, HER 2, HER 2/NEU, Herstatin, Human epidermal growth factor receptor 2, Metastatic lymph node gene 19 protein, MLN 19, MLN19, NEU, NEU proto oncogene, Neuro/glioblastoma derived oncogene homolog, Neuroblastoma/glioblastoma derived oncogene homolog, NGL, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase type cell surface receptor HER2, Tyrosine kinase-type cell surface receptor HER2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog), V erb b2 avian erythroblastic leukemia viral oncoprotein 2, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573) - Antibodies.comEnlarge Image
Figure 1: Western Blot - HER2 (phospho Tyr1248) Cell Based ELISA Kit (A103792)
Western blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 30', using Anti-HER2 (phospho Tyr1248) Antibody.
Protocol Diagram - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573) - Antibodies.comEnlarge Image
Figure 2: HER2 (phospho Tyr1248) Cell Based ELISA Kit (A103792)
A diagram showing the protocol for HER2 (phospho Tyr1248) Cell Based ELISA Kit.
Protocol Summary - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573) - Antibodies.comEnlarge Image
Figure 3: HER2 (phospho Tyr1248) Cell Based ELISA Kit (A103792)
A summary of the protocol for HER2 (phospho Tyr1248) Cell Based ELISA Kit.
Western Blot - Control Antibody - HER2 (phospho Tyr1248) Cell Based ELISA Kit (FLUO-CBP1573) - Antibodies.comEnlarge Image
Figure 4: Western Blot - HER2 (phospho Tyr1248) Cell Based ELISA Kit (A103792)
The mouse monoclonal antibody to GAPDH is used as a positive control in HER2 (phospho Tyr1248) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Publishing research using HER2 (phospho Tyr1248) Cell Based ELISA Kit (A103792)? Please let us know so that we can list the citation on this page.

Alternative products to HER2 (phospho Tyr1248) Cell Based ELISA Kit (A103792)

Western Blot - HER2 (phospho Tyr1248) Cell Based ELISA Kit (CBP1573) - Antibodies.com
(4)
A102161
HER2 (phospho Tyr1248) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - HER2 Cell Based ELISA Kit (CB5329) - Antibodies.com
(4)
A103181
HER2 Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - HER2 (phospho Tyr1221 + Tyr1222) Cell Based ELISA Kit (CBP1572) - Antibodies.com
(4)
A102234
HER2 (phospho Tyr1221 + Tyr1222) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - HER2 (phospho Tyr877) Cell Based ELISA Kit (CBP1574) - Antibodies.com
(4)
A102334
HER2 (phospho Tyr877) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - HER2 (phospho Tyr1112) Cell Based ELISA Kit (CBP1724) - Antibodies.com
(4)
A102214
HER2 (phospho Tyr1112) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Antibodies.com Image Placeholder - Discover more for less
A102362
HER2 (phospho Thr686) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - HER2 (phospho Tyr1248) Cell Based ELISA Kit (CBP1573) - Antibodies.com
(4)
A102161
HER2 (phospho Tyr1248) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - HER2 Cell Based ELISA Kit (CB5329) - Antibodies.com
(4)
A103181
HER2 Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - HER2 (phospho Tyr1221 + Tyr1222) Cell Based ELISA Kit (CBP1572) - Antibodies.com
(4)
A102234
HER2 (phospho Tyr1221 + Tyr1222) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - HER2 (phospho Tyr877) Cell Based ELISA Kit (CBP1574) - Antibodies.com
(4)
A102334
HER2 (phospho Tyr877) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Western Blot - HER2 (phospho Tyr1112) Cell Based ELISA Kit (CBP1724) - Antibodies.com
(4)
A102214
HER2 (phospho Tyr1112) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
Antibodies.com Image Placeholder - Discover more for less
A102362
HER2 (phospho Thr686) Cell Based ELISA Kit
Suitable for adherent cells and suspension cells.
See all HER2 ELISA Kits
Main Menu Contact Us 0Checkout
Top