HER2 Cell Based ELISA Kit (A103181)

Name: HER2 Cell Based ELISA Kit
Brand: Antibodies.com
SKU: A103181
Price: 550.0 USD
Availability: InStock

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$550

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Freight Charges

$40 to United States

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5-8 Business Days

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Specifications

Name

HER2 Cell Based ELISA Kit

Assay Type

Cell-based (qualitative)

Principle of Assay

This HER2 Cell Based ELISA Kit allows for the detection of HER2 and the effects that certain stimulation conditions have on HER2 expression in different cell lines. Qualitative determination of HER2 concentration is achieved by an indirect ELISA format. In essence, the HER2 is captured by Anti-HER2 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this HER2 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Adherent cells and suspension cells.

Product Range

> 5000 Cells

Assay Time

4 hours 30 minutes

Reactivity

Human, Mouse, Rat

Recovery

Storage

Store at + 4°C. Stable for 6 months.

Synonyms

C erb B2/neu protein, CD340, CD340 antigen, Cerb B2/neu protein, CerbB2, Erb b2 receptor tyrosine kinase 2, ErbB 2, ERBB2, ERBB2_HUMAN, HER 2, HER 2/NEU, Herstatin, Human epidermal growth factor receptor 2, Metastatic lymph node gene 19 protein, MLN 19, MLN19, NEU, NEU proto oncogene, Neuro/glioblastoma derived oncogene homolog, Neuroblastoma/glioblastoma derived oncogene homolog, NGL, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase type cell surface receptor HER2, Tyrosine kinase-type cell surface receptor HER2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog), V erb b2 avian erythroblastic leukemia viral oncoprotein 2, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - HER2 Cell Based ELISA Kit (A103181)

Western blot analysis of extracts from HepG2 cells, using Anti-EPHB1/2 (Ab-594/604) Antibody.

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Figure 2: HER2 Cell Based ELISA Kit (A103181)

A diagram showing the protocol for HER2 Cell Based ELISA Kit.

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Figure 3: HER2 Cell Based ELISA Kit (A103181)

A summary of the protocol for HER2 Cell Based ELISA Kit.

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Figure 4: Western Blot - HER2 Cell Based ELISA Kit (A103181)

The mouse monoclonal antibody to GAPDH is used as a positive control inHER2 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.

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