Anti-Hemoglobin subunit alpha Antibody [ARC1510] (A307978)

Name: Anti-Hemoglobin subunit alpha Antibody [ARC1510]
Brand: Antibodies.com
SKU: A307978
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1510] antibody to Hemoglobin subunit alpha for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Hemoglobin subunit alpha Antibody [ARC1510]

Description

Rabbit monoclonal [ARC1510] antibody to Hemoglobin subunit alpha.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hemoglobin subunit alpha (HBA1) (HBA1) (P69905).

Sequence

MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1510

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

11 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Alpha 1 globin, Alpha globin, Alpha one globin, Alpha-globin, HBA1, HBA2, HBA_HUMAN, Hemoglobin alpha 1, Hemoglobin alpha 1 chain, Hemoglobin alpha 1 globin chain, Hemoglobin alpha 2, Hemoglobin alpha chain, MGC126895, MGC126897

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Hemoglobin subunit alpha Antibody [ARC1510] (A307978)

Western blot analysis of extracts of Rat liver, using Anti-Hemoglobin subunit alpha Antibody [ARC1510] (A307978) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-Hemoglobin subunit alpha Antibody [ARC1510] (A307978)

Immunohistochemistry analysis of paraffin-embedded rat kidney red blood cells using Anti-Hemoglobin subunit alpha Antibody [ARC1510] (A307978) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Hemoglobin subunit alpha Antibody [ARC1510] (A307978)

Immunohistochemistry analysis of paraffin-embedded blood cell staining in human placenta tissue using Anti-Hemoglobin subunit alpha Antibody [ARC1510] (A307978) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Hemoglobin subunit alpha Antibody [ARC1510] (A307978)

Immunohistochemistry analysis of paraffin-embedded blood cell staining in mouse lung tissue using Anti-Hemoglobin subunit alpha Antibody [ARC1510] (A307978) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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