This antibody recognises human heme oxygenase-1 (HO-1), a 288 amino acid enzyme with a crucial role in heme catabolism, cleaving the heme ring to form biliverdin which is further processed to form bilirubin by a reductase enzyme. HO-1 activity is highest in the spleen where it is involved in the processing of senescent erythrocytes. HO-1 expression can be increased during cellular stress, including heat shock. Baseline levels of expression are high in certain tissues, including spleen and liver. Enriched enzyme preparations are recommended for experimental analysis.
Applications
WB, IHC-Fr
Dilutions
IHC-Fr: 1:50 - 1:1,000, WB: 1:1,000
Reactivity
Human, Bovine, Mouse, Rat, Pig
Cross Reactivity
This antibody does not cross react with Dog
Immunogen
Synthetic peptide sequence HDLSEALKEATKEVHC corresponding to amino acids 12-25 (+C-NH2 of human heme oxygenase 1.
Sequence
HDLSEALKEATKEVHC
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.065% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Human heme oxygenase 1 antibody recognizes human heme oxygenase-1 (HO-1), a 288 amino acid enzyme with a crucial role in heme catabolism, cleaving the heme ring to form biliverdin which is further processed to form bilirubin by a reductase enzyme. HO-1 activity is highest in the spleen where it is involved in the processing of senescent erythrocytes (Delaby et al. 2012). HO-1 expression can be increased during cellular stress, including heat shock (Ewing et al. 1992). Baseline levels of expression are high in certain tissues, including spleen and liver. Enriched enzyme preparations are recommended for experimental analysis.