Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770)

Name: Anti-Heme Oxygenase 1 Antibody [6B8-2F2]
Brand: Antibodies.com
SKU: A304770
Price: 520.0 USD
Availability: InStock

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$520

Mouse monoclonal [6B8-2F2] antibody to Heme Oxygenase 1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Heme Oxygenase 1 Antibody [6B8-2F2]

Description

Mouse monoclonal [6B8-2F2] antibody to Heme Oxygenase 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with HO-2.

Immunogen

His-tagged Rat HO-1.

Host

Mouse

Clonality

Monoclonal

Clone ID

6B8-2F2

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

HMOX1, HO, HO-1, HO1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770)

Western blot analysis of Human, mouse, rat Rat kidney Lysate showing detection of ~31 kDa HO-1 protein using Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat kidney Lysate. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was HRP Goat Anti-Mouse at 1:50 for 1 hour at room temperature. Color Development: TMB solution for 5 minutes at room temperature. Predicted/Observed Size: ~31 kDa.

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Immunofluorescence - Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770)

Immunocytochemistry/Immunofluorescence analysis of mouse fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Heme Oxygenase 1 Antibody [6B8-2F2] (A304770), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus, Cytoplasm. Magnification: 60X.

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