Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Cell-cell border staining in epidermis, punctuate nuclear staining..
Western Blot - Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771)
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of HO-1 protein using Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Microsome. Endoplasmic reticulum. Localizes to the nucleus upon hypoxia. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: muscle, dermis, hair follicles, epidermis: nuclear everywhere and some cytoplasmic staining.
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Heme Oxygenase 1 Antibody [1F12-A6] (A304771), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Microsome. Endoplasmic reticulum. Localizes to the nucleus upon hypoxia. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite.
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