Anti-HDAC6 Antibody [ARC0805] (A307941)

Name: Anti-HDAC6 Antibody [ARC0805]
Brand: Antibodies.com
SKU: A307941
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0805] antibody to HDAC6 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-HDAC6 Antibody [ARC0805]

Description

Rabbit monoclonal [ARC0805] antibody to HDAC6.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC6 (Q9UBN7).

Sequence

MTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDEQLNEFHCL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0805

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CPBHM, FLJ16239, HD 6, HD6, HDAC 6, HDAC6_HUMAN, Histone deacetylase 6, Histone deacetylase 6 (HD6), JM 21, JM21, KIAA0901, OTTHUMP00000032398, OTTHUMP00000197663, PPP1R90, Protein phosphatase 1 regulatory subunit 90

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-HDAC6 Antibody [ARC0805] (A307941)

Western blot analysis of extracts of various cell lines, using Anti-HDAC6 Antibody [ARC0805] (A307941) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-HDAC6 Antibody [ARC0805] (A307941)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-HDAC6 Antibody [ARC0805] (A307941) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-HDAC6 Antibody [ARC0805] (A307941)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-HDAC6 Antibody [ARC0805] (A307941). This Western blot was performed on the immunoprecipitate using Anti-HDAC6 Antibody [ARC0805] (A307941) at a dilution of 1:1000.

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