The antibody detects endogenous level of HDAC4 / HDAC5 / HDAC9 only when phosphorylated at serine 246 / 259 / 220.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 246 / 259 / 220 (T-A-S(p)-EP) derived from Human HDAC4 / HDAC5 / HDAC9.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Predicted MW
140 124 111 kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
AHO3, BDMR, EC 3.5.1.98, HA6116, HD 4, HD4, HDAC 4, HDAC A, HDAC4_HUMAN, HDACA, Histone deacetylase 4, Histone Deacetylase A, KIAA0288
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9 (Phospho-Ser246/259/220) Antibody #11517 (left) or the same antibody preincubated with blocking peptide (right).
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