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Anti-HDAC2 (Phospho-Ser394) Antibody (A50180)

Western blot - HDAC2 (Phospho-Ser394) Antibody from Signalway Antibody (11191) - Antibodies.com Immunohistochemistry - HDAC2 (Phospho-Ser394) Antibody from Signalway Antibody (11191) - Antibodies.com Immunofluorescence - HDAC2 (Phospho-Ser394) Antibody from Signalway Antibody (11191) - Antibodies.com Western blot - HDAC2 (Phospho-Ser394) Antibody from Signalway Antibody (11191) - Antibodies.com
$265

Rabbit polyclonal antibody to HDAC2 (Phospho-Ser394) for WB, IHC and IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-HDAC2 (Phospho-Ser394) Antibody
Description
Rabbit polyclonal antibody to HDAC2 (Phospho-Ser394)
Specificity
The antibody detects endogenous level of HDAC2 only when phosphorylated at serine 394.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 394 (E-D-S(p)-G-D) derived from Human HDAC2.
Host
Rabbit
Clonality
Polyclonal
Conjugate

Unconjugated

Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Predicted MW
60kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
D10Wsu179e, HD 2, HD2, HDAC 2, HDAC2_HUMAN, Histone deacetylase 2, Histone deacetylase 2 (HD2), OTTHUMP00000017046, OTTHUMP00000227077, OTTHUMP00000227078, RPD3, transcriptional regulator homolog RPD3, YAF1, YY1 associated factor 1, YY1 transcription factor binding protein, Yy1bp
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western blot - HDAC2 (Phospho-Ser394) Antibody from Signalway Antibody (11191) - Antibodies.comEnlarge Image
Figure 1: Anti-HDAC2 (Phospho-Ser394) Antibody (A50180)
Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) Antibody #11191.
Immunohistochemistry - HDAC2 (Phospho-Ser394) Antibody from Signalway Antibody (11191) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-HDAC2 (Phospho-Ser394) Antibody (A50180)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) Antibody #11191 (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence - HDAC2 (Phospho-Ser394) Antibody from Signalway Antibody (11191) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-HDAC2 (Phospho-Ser394) Antibody (A50180)
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear staining using HDAC2 (Phospho-Ser394) Antibody #11191.
Western blot - HDAC2 (Phospho-Ser394) Antibody from Signalway Antibody (11191) - Antibodies.comEnlarge Image
Figure 4: Anti-HDAC2 (Phospho-Ser394) Antibody (A50180)
Western blot analysis of extracts from HepG2 cells, treated with calf intestinal phosphatase (CIP), using HDAC2 (Phospho-Ser394) Antibody #11191.
Publishing research using Anti-HDAC2 (Phospho-Ser394) Antibody (A50180)? Please let us know so that we can list the citation on this page.

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