Anti-HCN1 Antibody [S70] (A304768)

Name: Anti-HCN1 Antibody [S70]
Brand: Antibodies.com
SKU: A304768
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S70] antibody to HCN1 for WB, IHC, IP and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-HCN1 Antibody [S70]

Description

Mouse monoclonal [S70] antibody to HCN1.

Applications

WB, IHC, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with HCN2.

Immunogen

Fusion protein amino acids 778-910 (C terminus) of rat HCN1.

Host

Mouse

Clonality

Monoclonal

Clone ID

S70

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BCNG-1, BCNG1, Brain cyclic nucleotide gated channel 1, Brain cyclic nucleotide-gated channel 1, HAC 2, HAC2, HCN1_HUMAN, Hyperpolarization activated cyclic nucleotide gated potassium channel 1, Potassium channel, voltage-gated, brain, 1, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Immunofluorescence - Anti-HCN1 Antibody [S70] (A304768)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-HCN1 Antibody [S70] (A304768), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN1 Antibody (D) Composite.

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Figure 2: Immunohistochemistry - Anti-HCN1 Antibody [S70] (A304768)

Immunohistochemistry analysis of mouse frozen brain section, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-HCN1 Antibody [S70] (A304768) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature.

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Figure 3: Immunohistochemistry - Anti-HCN1 Antibody [S70] (A304768)

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Figure 4: Immunohistochemistry - Anti-HCN1 Antibody [S70] (A304768)

Immunohistochemistry analysis of human hippocampus, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HCN1 Antibody [S70] (A304768) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.