Anti-Haptoglobin Antibody [E07-10E9] (A280291)

Anti-Haptoglobin Antibody [E07-10E9]

Mouse monoclonal [E07-10E9] antibody to Haptoglobin.

This antibody recognises haptoglobin, an alpha sialoglycoprotein. Synthesis of haptoglobin is induced by IL-6, IL-1 beta and TNF and it is produced as a pro-protein before it is cleaved in the endoplasmic reticulum by C1r-like protein, to generate the mature form. Haptoglobin is composed of two peptide chains referred to as the alpha and beta chains. It is largely expressed by hepatocytes, but is also expressed to a lesser extent in lung, skin and kidney tissues. A key role of haptoglobin is its hemoglobin binding capacity, which is utilized during hemolysis to prevent iron loss and subsequent kidney damage. The haptoglobin-hemoglobin complex is removed by the CD163 receptor expressed on the surface of monocytes and macrophages. Haptoglobin also plays roles in the innate and adaptive immune system and has anti-inflammatory activity such as inhibiting monocyte production of TNF, IL-11 and IL-12. The purified Mouse anti Human haptoglobin antibody, clone E07-10E9 (MCA6234GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human haptoglobin antibody, clone C08-1E10 (MCA6235B) as the detection antibody.

ELISA: 1 µg/ml

Human

Native haptoglobin purified from human plasma.

Mouse

Monoclonal

E07-10E9

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human haptoglobin antibody, clone E07-10E9, recognizes haptoglobin, an alpha sialoglycoprotein. Synthesis of haptoglobin is induced by IL-6, IL-1 beta and TNF and it is produced as a pro-protein before it is cleaved in the endoplasmic reticulum by C1r-like protein, to generate the mature form (Redmond et al. 2018). Haptoglobin is composed of two peptide chains referred to as the alpha and beta chains. It is largely expressed by hepatocytes, but is also expressed to a lesser extent in lung, skin and kidney tissues (Vanuytsel et al. 2013). A key role of haptoglobin is its hemoglobin binding capacity, which is utilized during hemolysis to prevent iron loss and subsequent kidney damage. The haptoglobin-hemoglobin complex is removed by the CD163 receptor expressed on the surface of monocytes and macrophages (Sadrzadeh & Bozorgmehr 2004). Haptoglobin also plays roles in the innate and adaptive immune system and has anti-inflammatory activity such as inhibiting monocyte production of TNF, IL-11 and IL-12 (Arredouani et al. 2005).The purified Mouse anti Human haptoglobin antibody, clone E07-10E9 (MCA6234GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human haptoglobin antibody, clone C08-1E10 (MCA6235B) as the detection antibody.

HP, Zonulin