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Recombinant Anti-HA Tag Antibody [RM305] (Biotin) (A121320)

Western Blot - Anti-HA Tag Antibody (Biotin) [RM305] (A121320) - Antibodies.com Immunohistochemistry - Anti-HA Tag Antibody (Biotin) [RM305] (A121321) - Antibodies.com
Recombinant Antibody
$510

Recombinant rabbit monoclonal [RM305] antibody to HA Tag (Biotin) for WB, IP, ICC, Flow Cytometry and IHC.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-HA Tag Antibody [RM305] (Biotin)
Description
Recombinant rabbit monoclonal [RM305] antibody to HA Tag (Biotin).
Specificity
This antibody recognizes recombinant proteins containing a HA Tag, fused to either the amino or carboxy terminus.
Applications
WB, IP, ICC, Flow Cytometry, IHC
Dilutions
WB: 0.1-1 µg/ml, IP: 0.5-2 µg/ml, ICC: 0.1-1 µg/ml, FC: 0.1-1 µg/ml, IHC: 0.01-0.5 µg/ml
Cross Reactivity
There is no cross reactivity with other endogenous protein.
Immunogen
Synthetic peptide corresponding to HA Tag. Sequence is identical to Human Influenza Hemagglutinin (a.a. 98-106).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM305
Isotype
IgG
Conjugate

Biotin

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-HA Tag Antibody (Biotin) [RM305] (A121320) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-HA Tag Antibody [RM305] (Biotin) (A121320)
Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding KRas fusion protein (HA tag), using Anti-HA Tag Antibody [RM305] (Biotin) at 0.1µg/ml.
Immunohistochemistry - Anti-HA Tag Antibody (Biotin) [RM305] (A121321) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-HA Tag Antibody [RM305] (Biotin) (A121320)
Immunohistochemical staining of formalin fixed and paraffin embedded LNCaP cells overexpressing Bag1 protein (HA tag), using Anti-HA Tag Antibody [RM305] (Biotin) at 0.01µg/ml.
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