Western blot analysis of 293T, using Anti-HA Tag Antibody (A93157) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunofluorescence - Anti-HA Tag Antibody (A93157)
Immunofluorescence analysis of 293T-HA-C and 293T-HA-N cells using Anti-HA Tag Antibody (A93157) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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