This antibody recognises the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors.Rabbit anti HA-Tag antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting.In Western blotting of bacterial extracts Rabbit anti HA-Tag antibody has been shown not to cross-react with any endogenous proteins.
Applications
WB, IHC-P, ELISA
Reactivity
Viral
Immunogen
Synthetic peptide sequence YPYDVPDYA corresponding to amino acids 114-122 of the Epitope tag from haemagglutinin influenza, conjugated to Keyhole Limpet Haemocyanin. A cysteine residue was used to facilitate coupling at the C-terminus end.
Sequence
YPYDVPDYA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti HA-Tag antibody recognizes the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors.Rabbit anti HA-Tag antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting.In Western blotting of bacterial extracts Rabbit anti HA-Tag antibody has been shown not to cross-react with any endogenous proteins.